本片根据IDEA FACTORY于08年9月发行的PS2女性向恋爱冒险游戏《薄樱鬼——新选组奇谭》改编。动乱的幕末时期,雪村千鹤是在江户长大的兰学医者雪村纲道的女儿.父亲纲道原本应该是与女儿分开后在京都工作的。可是父亲突然神秘失踪,因为联络不上父亲而担心的千鹤决定女扮男装前往京都探访。在那里,她所看见的是,与饥渴地寻求鲜血的妖怪所战斗并制伏他们的新选组的队士们。千鹤与新选组结下了奇妙缘分,并因缘际会之下加入了新选组。在搜索失踪父亲的主人公面前,出现了神秘的刺客们,逐渐明朗化地新选组的秘密——罗刹的存在。在狂暴的时代中,在幕末争斗的背后,胸中挥舞着理想与信念之刃的男人们,与黑暗的战斗也拉开了序幕......
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